Publications

• Zawia A, Arnold ND, West L, Pickworth JA, Turton H, Iremonger J, Braithwaite AT, Cañedo J, Johnston SA, Thompson AAR, Miller G, Lawrie A (2020). Altered Macrophage Polarization Induces Experimental Pulmonary Hypertension and Is Observed in Patients With Pulmonary Arterial Hypertension. Atherosclerosis, Thrombosis and Vascular Biology, https://doi.org/10.1161/ATVBAHA.120.314639 

• Gibson JF, Prajsnar TK, Hill CJ, Tooke AK, Serba JJ, Tonge RD, Foster SJ, Grierson AJ, Ingham PW, Renshaw SA, & Johnston SA (2020). Neutrophils use selective autophagy receptor Sqstm1/p62 to target Staphylococcus aureus for degradation in vivo in zebrafish. Autophagy, 1-10. doi:10.1080/15548627.2020.1765521

• Prajsnar TK, Serba JJ, Dekker BM, Gibson JF, Masud S, Fleming A, Johnston SA, Renshaw SA, Meijer, AH (2020). The autophagic response to Staphylococcus aureus provides an intracellular niche in neutrophils. Autophagy, 1-15. doi:10.1080/15548627.2020.1739443

• Evans RJ & Johnston SA (2020) PPAR-gamma Fun(gi) With Prostaglandin. Nuclear Receptor Signaling, 17, 155076291989964-155076291989964.  

• Jones D, Hagen F, Donaldson C, Rudman J, Canedo J & Johnston SA (2019) Investigating a novel commensal strain of Candida famata within Daniorerio. Access Microbiology, 1(9).  

• Rudman J, Evans RJ & Johnston SA (2019) Are macrophages the heroes or villains during cryptococcosis?. Fungal Genetics and Biology, 132, 103261-103261.  

• Roddie HG, Armitage EL, Coates JA, Johnston SA & Evans IR (2019) Simu-dependent clearance of dying cells regulates macrophage function and inflammation resolution. PLOS Biology, 17(5). View this article in WRRO  

• Evans RJ, Pline K, Loynes CA, Needs S, Aldrovandi M, Tiefenbach J, Bielska E, Rubino RE, Nicol CJ, May RC , Krause HM et al (2019) 15-keto-prostaglandin E2 activates host peroxisome proliferator-activated receptor gamma (PPAR-γ) to promote Cryptococcus neoformans growth during infection. PLoS Pathogens, 15(3). View this article in WRRO  

• Duvenage L, Walker LA, Bojarczuk A, Johnston SA, MacCallum DM, Munro CA & Gourlay CW (2019) Inhibition of Classical and Alternative Modes of Respiration in Candida albicans Leads to Cell Wall Remodeling and Increased Macrophage Recognition.. mBio, 10(1). View this article in WRRO  

• Fehri H, Gooya A, Lu Y, Meijering E, Johnston SA & Frangi AF (2019) Bayesian Polytrees with Learned Deep Features for Multi-Class Cell Segmentation. IEEE Transactions on Image Processing, 1-1. View this article in WRRO  

• Probert M, Zhou X, Goodall M, Johnston SA, Bielska E, Ballou ER & May RC (2019) A glucuronoxylomannan epitope exhibits serotype-specific accessibility and redistributes towards the capsule surface during Titanisation of the fungal pathogen Cryptococcus neoformans.. Infection and Immunity. View this article in WRRO  

• Gow NAR, Amin T, McArdle K, Brown AJP, Brown GD, Warris A & the WTSA-MMFI Consortium (2018) Strategic Research Funding: A Success Story for Medical Mycology. Trends in Microbiology. View this article in WRRO  

• Watkins RA, Andrews A, Wynn C, Barisch C, King JS & Johnston SA (2018) Cryptococcus neoformans escape from Dictyostelium amoeba by both WASH-mediated constitutive exocytosis and vomocytosis. Frontiers in Cellular and Infection Microbiology, 8. View this article in WRRO  

• van Leeuwen LM, Evans RJ, Jim KK, Verboom T, Fang X, Bojarczuk A, Malicki J, Johnston SA & van der Sar AM (2018) A transgenic zebrafish model for the in vivo study of the blood and choroid plexus brain barriers using claudin 5. Biology Open, 7(2), bio030494-bio030494. View this article in WRRO  

• Ballou ER & Johnston SA (2017) The cause and effect of Cryptococcus interactions with the host. Curr Opin Microbiol, 40, 88-94. View this article in WRRO  

• Watkins RA, King JS & Johnston SA (2017) Nutritional Requirements and Their Importance for Virulence of Pathogenic Cryptococcus Species. Microorganisms, 5(4). View this article in WRRO  

• Gilbert AS, Seoane PI, Sephton-Clark P, Bojarczuk A, Hotham R, Giurisato E, Sarhan AR, Hillen A, Velde GV, Gray NS , Alessi DR et al (2017) Vomocytosis of live pathogens from macrophages is regulated by the atypical MAP kinase ERK5. Science Advances, 3(8). View this article in WRRO  

• Farrer RA, Voelz K, Henk DA, Johnston SA, Fisher MC, May RC & Cuomo CA (2016) Microevolutionary traits and comparative population genomics of the emerging pathogenic fungus Cryptococcus gattii. Philosophical Transactions of the Royal Society B: Biological Sciences, 371(1709), 20160021-20160021. View this article in WRRO  

• Evans RJ, Voelz K, Johnston SA & May RC (2016) Using Flow Cytometry to Analyze Cryptococcus Infection of Macrophages. Methods Mol Biol, 1519, 349-357. View this article in WRRO  

• Dee CT, Nagaraju RT, Athanasiadis EI, Gray C, Fernandez Del Ama L, Johnston SA, Secombes CJ, Cvejic A & Hurlstone AF (2016) CD4-Transgenic Zebrafish Reveal Tissue-Resident Th2- and Regulatory T Cell–like Populations and Diverse Mononuclear Phagocytes. Journal of Immunology, 197(9), 3520-3530. View this article in WRRO  

• Buckley CM, Gopaldass N, Bosmani C, Johnston SA, Soldati T, Insall RH & King JS (2016) WASH drives early recycling from macropinosomes and phagosomes to maintain surface phagocytic receptors. Proc Natl Acad Sci, 113(40), E5906-E5915. View this article in WRRO  

• Bojarczuk A, Miller KA, Hotham R, Lewis A, Ogryzko NV, Kamuyango AA, Frost H, Gibson RH, Stillman E, May RC , Renshaw SA et al (2016) Cryptococcus neoformans Intracellular Proliferation and Capsule Size Determines Early Macrophage Control of Infection. Scientific Reports, 6(1), 21489-21489. View this article in WRRO  

• Johnston SA, Voelz K & May RC (2016) Cryptococcus neoformans Thermotolerance to Avian Body Temperature Is Sufficient For Extracellular Growth But Not Intracellular Survival In Macrophages. Scientific Reports, 6. View this article in WRRO  

• Jubrail J, Morris P, Bewley MA, Stoneham S, Johnston SA, Foster SJ, Peden AA, Read RC, Marriott HM & Dockrell DH (2015) Inability to sustain intraphagolysosomal killing of Staphylococcus aureus predisposes to bacterial persistence in macrophages. Cellular Microbiology, 18(1), 80-96. View this article in WRRO  

• Gibson JF & Johnston SA (2015) Immunity to Cryptococcus neoformans and C. gattii during cryptococcosis. Fungal Genetics and Biology, 78, 76-86. View this article in WRRO  

• Voelz K, Johnston SA, Smith LM, Hall RA, Idnurm A & May RC (2014) ‘Division of labour’ in response to host oxidative burst drives a fatal Cryptococcus gattii outbreak. Nature Communications, 5, 5194-5194. View this article in WRRO  

• Sabiiti W, Robertson E, Beale MA, Johnston SA, Brouwer AE, Loyse A, Jarvis JN, Gilbert AS, Fisher MC, Harrison TS , May RC et al (2014) Efficient phagocytosis and laccase activity affect the outcome of HIV-associated cryptococcosis. Journal of Clinical Investigation, 124(5), 2000-2008.  

• Johnston SA & May RC (2013) Cryptococcus interactions with macrophages: evasion and manipulation of the phagosome by a fungal pathogen. Cell Microbiol, 15(3), 403-411.  

• Carnell M, Zech T, Calaminus SD, Ura S, Hagedorn M, Johnston SA, May RC, Soldati T, Machesky LM & Insall RH (2011) Actin polymerization driven by WASH causes V-ATPase retrieval and vesicle neutralization before exocytosis. Journal of Cell Biology, 193(5), 831-839.  

• Chayakulkeeree M, Johnston SA, Oei JB, Lev S, Williamson PR, Wilson CF, Zuo X, Leal AL, Vainstein MH, Meyer W , Sorrell TC et al (2011) SEC14 is a specific requirement for secretion of phospholipase B1 and pathogenicity of Cryptococcus neoformans. Molecular Microbiology, 80(4), 1088-1101.  

• Johnston SA & May RC (2010) The human fungal pathogen Cryptococcus neoformans escapes macrophages by a phagosome emptying mechanism that is inhibited by arp2/3 complex- mediated actin polymerisation. PLoS Pathogens, 6(8), 27-28.  

• Voelz K, Johnston SA, Rutherford JC & May RC (2010) Automated analysis of cryptococcal macrophage parasitism using GFP-tagged cryptococci. PLoS One, 5(12), e15968.  

• Ma H, Hagen F, Stekel DJ, Johnston SA, Sionov E, Falk R, Polacheck I, Boekhout T & May RC (2009) The fatal fungal outbreak on Vancouver Island is characterized by enhanced intracellular parasitism driven by mitochondrial regulation. Proc Natl Acad Sci, 106(31), 12980-12985.  

• Johnston SA, Bramble JP, Yeung CL, Mendes PM & Machesky LM (2008) Arp2/3 complex activity in filopodia of spreading cells. BMC Cell Biol, 9, 65.  

• Machesky LM & Johnston SA (2007) MIM: a multifunctional scaffold protein. J Mol Med (Berl), 85(6), 569-576.  

• Legg JA, Bompard G, Dawson J, Morris HL, Andrew N, Cooper L, Johnston SA, Tramountanis G & Machesky LM (2007) N-WASP involvement in dorsal ruffle formation in mouse embryonic fibroblasts. Mol Biol Cell, 18(2), 678-687.  

Publications In print

Rudman J, Marriott HM, Carlin LM & Johnston SA () The mouse lung early cellular innate immune response is not sufficient to control fungal infection with Cryptococcus neoformans. 

Conference proceedings 

Fehri H, Gooya A, Johnston SA & Frangi AF (2017) Multi-class Image Segmentation in Fluorescence Microscopy Using Polytrees (pp 517-528) View this article in WRRO